Dr. Marlene Siegel          Dr. Kathy Backus




On-Demand for 365 days


It can be done on your schedule
It requires no travel
Receive a certificate in the mail upon completion (US only)


O3Vets is the only company in North America dedicated to veterinary ozone therapy so you literally will not find this information anywhere else. If you do, it has probably been copied from us. Our recorded online training is a comprehensive, on-demand course that was filmed at one of our live training classes. This has been broken into two parts that can be purchased separately:

Basic Veterinary Oxone Therapy Course
Prolo + Ozone Injections Course

It consists of both PowerPoint presentations as well as protocols and hands-on training.


Pet Owners
All Others

Although anyone can purchase them, these courses will be most helpful for veterinary professionals. We are working to produce pet owner specific courses.


Margo Roman, DVM is owner of Main Street Animal Services of Hopkinton since 1983. She graduated from Tuskegee University School of Vet Medicine in 1978 and has now performed over 14,000 ozone therapy treatments. She has been certified as an ozone therapist through the American Academy of Ozone Therapy as well as the Society of Progressive Medical Education and has trained with O3Vets over the past few years. 

Kathy BackusDVM graduated from The Ohio State University School of Veterinary Medicine and has practiced integrative medicine for over twenty-four years. She is a certified ozone therapist and has utilized ozone therapy for over twelve years as the backbone of her practice in Utah. She has utilized dozens of tools over her years and has a comprehensive view of integrative veterinary medicine

Marlene Siegel, DVM is fast becoming the voice of ozone therapy. With decades of experience as an integrative veterinarian, she has empowered pets and pet parents to gain control of their own health. Dr. Siegel is an award-winning author, teacher, keynote speaker and out-of-the-box thinker. She incorporates more than 30 modalities into her clinic and has also been featured in the documentary, "The Truth About Cancer Pets". 

Jonathan Lowe founded O3Vets in 2014 to bring ozone therapy to the animal kingdom. He has developed and taught courses on ozone therapy for animals all over the world. In 2018 his company received the innovation award from the IVC Journal for its work in ozone therapy. He is currently writing a book on ozone therapy for animals with the hopes of providing better, more accessible information to veterinarians and pet owners




1. Class Overview

2. Oxidative Therapy
  A.What it is

3. Ozone Overview
  A. Why use it
  B. Natural formation of ozone
  C. Synthetic formation of ozone
  D. Industrial uses
  E. Dangers of ozone
  F. Half-Life
  G. Concentrations

4. Methods of Administration
  B. Limb-Bagging
  C. Insufflation Techniques
  D. Fluids
  E. Oils

5. Medical History

6. Indications
  A. Categories
  B. Specific Areas

7. Contraindications

8. Ozone Videos
  A. Pain Management

9. Biological & Clinical Effects
  A. Main Effect
  B. Secondary Effects
  C. Bocci Mechanism of Action

10. Major Works
  A. Bocci
  B. The Low-Dose Ozone Concept
  C. ISCO3
  D. The Madrid Declaration
  E. Ozone Therapy in Practice Manual

11. Ozone Studies
  A. Zotero
  C. Books

12. Prolotherapy + Ozone
  A. Indications and Applications
  B. Solution
  C. Case Study

13. Equipment
  A. What’s needed
  B. Setup
  C. Ozone resistance

14. Client Education
  A. Available Materials
  B. Cost vs. Value

15. Ozone Oils
  A. Trustworthy sources
  B. Uses
  C. Limitations

16. O3UV Combination Therapy
  A. UltraImmunotherapy
  B. Medical Effects of light
  C. Combination of Ozone and Light
  D. Vasogen Studies

17. Clinical Applications & Observations

18. Hands-On Training


Can multiple people take this course?

FAQ We have a purchase option that will give each person in your entire clinic their own account and 365 days of access.

What courses are available and are you going to add more?

We have a basic course and a Prolo+Ozone course available. We welcome suggestions on what you would like to see in the future.

Are there tests?

Yes, we have a simple test at the end of each video section and also a more extensive test at the end of the course.

Is the information understandable and usable for a pet owner?

As a whole, yes it is. There are a few places where a rudimentary understanding of
biology and veterinary medicine will be helpful but, most pet owners will not be
able to implement a number of the administration methods that will be taught.