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Intramammry Application of Ozone Therapy to Acute Clinical Mastitis in Diary Cows.

MAHT for Ehrlichiosis – Canine

Changes in Haemochromocytometric value

Heart and Respiratory Rates – Equine

Hematological Variables – Equine

Intervertebral Lesions – Equine

Paravertebral Treatment – Equine

Skin healing – Equine

Spinal muscle disorder – Equine

Virus inactivation – Equine

O3UV  is one recommended method of administration, however, if just ozone applications is being administered, there are various ways to introduce it into the body depending on what is being treated.  Below you will find the various ways that ozone is currently administered to animals. Because of the differing size of animals these estimations of quantity may not apply.

Major Autohemotherapy (MAHT)

Major autohaemotherapy calls for the removal of between 10-250 ml of the patient’s blood. Ozone and oxygen are then injected into the blood, the ozonated blood is re-introduced into a vein. These methods have been used to treat a wide variety of health problems, including herpes, arthritis, cancer, heart disease. It is probably the most commonly used type of ozone therapy today.

Minor Autohemotherapy (mAHT) 

Minor autohaemotherapy involves removing a small amount (usually 3-10 ml) of the patient’s blood from a vein with a syringe. The blood is then treated with an equal amount of ozone and given back to the patient as an intramuscular or subcutaneous injection. The blood and ozone becomes a type of auto-vaccine, which can be very specific and effective.

Direct intra-arterial and intravenous application (DIV)

An ozone/oxygen mixture is slowly injected into an artery or vein with a hypodermic syringe. This method is used primarily for arterial circulatory disorders.  This can be used for large animals according to procedures developed by Judith Shoemaker DVM. 


A mixture of ozone and oxygen is introduced through the rectum and absorbed into the body through the intestine. Used for a wide variety of health problems, this method is considered one of the safest. In a typical treatment for ulcerative colitis, for example, 75 micrograms of ozone per milliliter of oxygen are used (treatment begins with 50ml of oxygen which can be increased slowly to 500 ml per treatment).

Ozonated water

This method calls for ozone gas to be bubbled through distilled water or saline.  The water is used externally to bathe wounds, burns and slow-healing skin infections. In Russia, physicians are using ozonated water to irrigate body cavities during surgery. In both Russia and Cuba, ozonated water is used to treat a wide variety of intestinal and gynecological problems, including ulcerative colitis, duodenal ulcers, gastritis, diarrhea and vulvovaginitis.  Because water doesn’t hold ozone well, it must be ozonated for about 20-30 minutes initially and then recharged every 20 minutes while it is being used.

Intra-articular injection (prolozone)

In this method, a specific mixture of procaine, vitamins, minerals and homeopathic remedies are injected directly into the joints. Ozone is also injected to the same site. It is used primarily by physicians throughout the world to treat joint pain, herniated discs, arthritis, and other joint diseases.

Intramammary infusion 

Instead of antibiotics, this can be very effective for mastitis in large animals.  The lack of side effects or residue in animals such as dairy cows makes it a perfect choice as it will not affect the quality of their milk as an antibiotic would.

Limb bagging

This non-invasive method uses a specially made plastic bag that is placed around the area to be treated. An ozone/oxygen mixture is pumped into the bag and the mixture is absorbed into the body through the skin. Ozone bagging is primarily recommended for treating leg ulcers, gangrene, fungal infections, burns and slow-healing wounds. 

Ozonated oil

Used primarily to treat skin problems, ozone gas is added to olive, hemp, sunflower or other oils and applied as a balm or salve for long term, low-dose exposure. Studies show a more rapid healing effect. 

Inhalation of ozone

Ozone can be bubbled through olive oil and the resulting atomized oil/ozone mix can be safely inhaled

Caution: The lungs are the organs most sensitive to direct ozone gas . It is not safe to breath high concentrations of ozone that have not been bubbled through an oil.

Ozone Indications and Treatment Chart

A whole number of pathological conditions exist which can be influenced positively or even healed by ozone. This is a fact which has been confirmed by a wide series of scientific investigations and medical publications. As a rule, medical ozone is applied in addition to other therapeutic methods ie it belongs to the field of complementary medicine.

As with all other forms of medical treatment, no 100 % guarantee can be given that ozone therapy will alleviate the condition it has been applied for. Success will vary according to the patient’s state of health, the frequency of ozone treatment, the doses and concentrations applied, and a number of other factors.


MAHT can be applied with great profit in the form of an additional, biological therapy in malignant conditions. Here we make use of ozone’s immune-activating properties, produced when it is applied at a low dose. Immune cells – such as lymphocytes, helper and suppressor cells and natural killer cells – are activated via biological reactions induced by ozone to respond by producing messenger proteins called cytokines to which, for example, the interferons belong. In fact, ozone makes the body produce increased quantities of its own interferons and interleukins. On reintroduction of the ozonated blood, a cascade of positive immune reactions are set off which also contribute to general health and wellbeing.

Viral Infections


Herpes are caused by viruses and can be treated very successfully with ozone in combination with other medical methods.
In the case of herpes, the complementary application of ozone is useful, both in the form of ozonized water and O3UV.

 Inflammatory processes of the liver

Inflammatory diseases of the liver are counted among the classical indications for medical ozone. Whereas the treatment of hepatitis A (HVA = hepatitis virus A) is relatively unproblematic and produces complete healing, another form, hepatitis B (HVB = hepatitis virus B), frequently takes a chronic course. Here, in addition to classical medical treatment methods, we find that ozone autohaemotransfusion or the rectal insufflation of controlled ozone/oxygen gas quantities can be successful. This also applies to the treatment of hepatitis C which, due to an incubation period capable of lasting for a number of years, is generally not diagnosed as a liver disease until it has become a chronic condition.


Bacterial/Fungal Infections

Fungal skin infections and infected skin lesions

The fungicidal and bactericidal properties of ozone have been successfully in use for over 100 years in the treatment of water for drinking. They make medical ozone an effective therapeutic agent in combating persistent skin moulds and fungi, especially those of the paws with bacterial infections, fungal infections of the trunk or against fungal/mycotic infections of the mucous membranes.

Infected wounds

The local treatment of infected wounds, such as may easily occur with open bed sores (decubitus), ulcers of the lower leg (ulcus cruris), diabetic gangrene or delayed/disturbed wound healing processes, belong to the classical application fields of medical ozone. Here we+ primarily make use of its disinfectant, ie bactericidal and fungicidal, effects, to obtain a germ-free and clean wound. Once this has been achieved, we then apply lower doses of O3 gas to accelerate/improve wound healing. 

Inflammatory Conditions

Chronic polyarthritis

The term arthritic/rheumatic conditions includes various painful diseases of the skeletal or muscular system, partly also involving functional restrictions. In general, the application of medical ozone can here be regarded as being a complementary measure only, combined with a basic classical method and corresponding physiotherapy. In the case of rheumatic arthritis (chronic polyarthritis), our experience shows that ozone autohaemotherapy is a very useful complementary form when given during the non-acute phases. Its immunomodulatory and antiinflammatory properties are here its basic principle of action.

When we divide inflammatory diseases of the joints into three phases, it is particularly stages 1 and 2, ie those not yet involving a severe bone deformation, that are responsive to medical ozone application. This applies to gonarthritis (inflammation of the knee joint) or the active arthritic form in knee and shoulder joints. Here, intraarticular ozone injections applied in addition to basic standard medical methods – in this case specific exercise therapy measures – are of great use. We here make full use of the antiinflammatory effects of ozone, in addition to its immunomodulatory properties and its ability to activate cartilage metabolism. 

Circulatory Conditions

Arterial circulatory disorders, for which, among other symptoms, a feeling of coldness in the legs or pains after walking only short distances (“short cut” syndrome) are alarming signs, have been a classical indication for ozone therapy for over 40 years now. Its success has been confirmed by a large number of clinical trials. Ozone is applied as a complement to and in combination with other methods in classical and complementary medicine.

Autoimmune Disorders


Respiratory Disorders


Other Conditions

Regeneration and revitalization

Animals that are exhibiting excessive mental and physical tension respond particularly well to O3 application. Its ability to activate red and white blood cell metabolism produces an improvement in general wellbeing, and brings about a general revitalization.

Older animals – Prevention and therapy

Animals that are advanced in years respond very well to medical ozone therapy.  There are multiple advantages  such as that of making an improved supply of oxygen accessible to all tissues, mobilizing the immune system, and activating the body’s own supply of antioxidants and radical scavengers.  Furthermore, we have its positive influence in cerebral circulatory disorders – a condition characterized by a general reduction in physical performance, insecurity in walking, dizziness.  In addition to other measures applied in complementary medicine, medical ozone is also used as a preventive, contributing to a marked increase in life quality.

Diseases of the eye

Circulatory disturbances due to old age also affect the eye with atrophic and degenerative changes. For example, senile macular degeneration is well known, occurring at the center of the retina – the point where visual focus is at its sharpest. Its sequels are thus able to influence the optic nerve to varying degrees, producing what is called optic nerve atrophy. Results obtained from a clinical trial carried out at the University of Siena show, in addition to reports from practical applications, improvements in vision lasting for 6 to 8 months after an MAHT (Major Auto Hemo Therapy).  Continuing the series of treatments can produce further improvement in visual performance or prevent it from worsening. 

Intestinal diseases – prostatic and colitis

In the case of inflammatory intestinal processes, particularly during their early stages, the local application of ozone in the form of rectal O3 gas insufflation has been proved very useful. A series of 10 ozone applications is sufficient in most cases. Several such series are only necessary in about 10 % of the patients (from a prostatic clinical study on O3 involving 248 patients).


Contraindications for ozone therapy are the following:
– acute and chronic tendency to bleedings;
– thrombocytopenia;
– hyperthyroidism;
– cramps in the anamnesis;
– individual intolerance to ozone;
– blood coagulation disorder;
Ozone should be used most carefully in case of decompensated conditions:
– in decompensated heart insufficiency;
– in severe acute toxication (incl. alcohol);
– in hypoglycemic conditions;
– in extremal sympathicotonic and parasympathicotonic reactions.
After myocardial infarction ozone therapy can be used not earlier than after 6 months, in patients with hemorrhagic insult in the anamnesis ozone therapy is not recommended for use.

Discovery History of ozone

Although ozone was discovered in 1785 by Dutch physicist Martinus Van Marum (1750-1837) as he perceived a peculiar odor that was generated near electrostatic machines, it was not until May 1840 that the German chemist Christian Friedrich Schönbein (1799-1868) synthesized it.


Christian Friedrich Schönbein

In 1857 Werner von Siemens built the first superior induction tube with which Kleinmann made the first attempt to destroy microorganisms and also performed the first gas insufflations in animals and humans. In 1870, the German doctor Lender published the first paper ever on practical biological effects relating to water disinfection. The discovery of the antimicrobial properties of ozone revolutionized medicine during this time as it would still be seventy years before the emergence of penicillin.
In 1873 Fox discovered the ability of this chemical agent for eliminating microorganisms. According to Dr. Kellogg in his book on diphtheria, there is evidence to its use as a disinfectant since 1881. This discovery crossed the ocean to North America and in 1885, the Florida Medical Association published the first textbook on medical applications of ozone.
In 1893, the first facility in Ousbaden (Netherlands) for the disinfection and purification of water for human consumption and waste was created. To date, there are more than 3,000 water treatment plants with ozone in the Netherlands alone. Zurich, Florence and other cities have been acquiring these systems to disinfect water.



Nikola Tesla (1856-1943) of Croatian origin and later on a US citizen, patented his first ozone generator in 1896 and in 1900 founded “Tesla Ozone Co., manufacturer of generators for medical use. Tesla was also the first to ozonate olive oil.

In 1898 Drs. Thauerkauf and Luth created the Institute of Oxygen-Ozone Therapy in Germany and publish the first studies in animals.

Medical Ozone in the XX Century

United States

The first American therapeutic use of ozone was by Dr. John H. Kellogg in ozone steam saunas at his Battle Creek, Michigan sanitarium in 1880 as he wrote in his book, Diptheria: Its Nature, Causes, Prevention and Treatment.

John Kellogg

In 1902, Dr. Charles Linder of Spokane, Washington was written up in an article in a local paper which stated that he had injected ozone as a part of his standard medical practice.

In 1904, “The Medical Uses of Hydrozone (ozonated water) and Glyzozone (ozonated olive oil) by Charles Marchand, a New York chemist, appeared in its 19th edition. Believe it or not, this book is in the Library of Congress with the US Sergeon General’s stamp of approval on it.

In 1911, A Working Manual of High Frequency Currents was published by Dr. Noble Eberhart, the head of the Dept. of Physiologic Therapeutics at Loyola University, Chicago. In Chapter 9, he details the use of zone to treat tuberculosis, anemia, chlorosis, tinnitus, wooping cough, asthma, bronchitis, hay fever, insomnia, pneumonia, diabetes, gout and syphilis.

In 1912, Dr. H.C. Bennett published Electro-Therapeutic Guide. In that publication, he described the use of Ozol (ozone breathed after running it through eucalyptus, pine or thyme oils).

In 1929, a book called Ozone and its Therapeutic Action was published in the US listing 114 diseases and how to treat them with ozone. Its 40 authors were leaders in many of the prominent hospitals of the time.

Finally, in 1933, the decline of a promising treatment began as the discovery of penicillin and insulin rocketed the drug market forward. Ozone and other treatments slowly lost favor as pharmaceutical giants heavy influence on the medical culture began a shift in power that still exists.

Today, there are hundreds of medical doctors and veterinarians here in the US who use ozone to treat their patients every day.

In 1913, Dr. Blass founded the first German association of ozone therapy. Meanwhile, the First World War in Europe had just exploded. In 1915, Dr. Wolf, chief surgeon of the medical services of the German army, extends its use for topical treatment of infected wounds, frozen foot, gangrene and decubitus ulcers.
in 1932, a Swiss dentist, Dr. Fish, published a paper on the applications of ozone in dentistry to treat caries, and also patented the first device specifically for this application, the Cytozon.
In 1935 Edwin Payr (Austrian-German) showed the wound healing effect of ozone. In Germany, after the outbreak of World War II, Dr. Wolf published “Medical Ozone”, which is considered THE classic book on ozone therapy.
Despite its obvious usefulness, ozone as a healing method for infectious diseases gradually fell into disuse. Moreover, in 1940, the U. S. Food and Drug Administration (FDA) sealed the fate of medical ozone generators by promoting their own pharmaceutical industry which was reinforced after the acquisition of a pharmaceutical giant, German Bayer.
The emergence of ozone resistant plastics lead German physician Dr. Hänsler to manufacture the first modern ozone generator in 1957.
In 1961 Dr. Hans Wolff introduced major and minor autohemotherapy into his practice and in the early 70’s the German Society of Ozone Therapy was created which contributed to the momentum of ozone therapy and its application in various diseases.

In the 70’s the first communications from the Russians on the successful use of ozone in burned patients appeared. At the same time, in Minsk (Belarus), the first patients were successfully treated for bronchial asthma with ozonated steam inhalations.
The most enthusiastic students and researchers of ozone in this part of the world, have been scientists, members of the Central Laboratory of Scientific Research of the State Academy of Medicine of Nizhny Novgorod (former Gorki), under the direction of the academician Ramn B. A. Koroleva. There, on October 1977, the first experiment on dogs with ozonated saline solution was performed.
As the Russians studied the potential of ozonated saline, they also began to develop new methods for the application of ozone such as the use of ozone in the preservation
of blood, blood ozonation and the infusion of ozonated saline during periods of post-operative and post-resuscitation. (Ozone Therapy Manual, Nizhny Novgorod, 2008. Translation from Russian into Spanish Adriana Schwartz).
In 2005, the Russians were the first to record the use of ozone in dermatology and cosmetology at state level. Two year later, in 2007, they also reported its use in gynecology-obstetrics, neonatology, traumatology and burn treatment. Russia became the first country in the world to regularize and implement ozone therapy at the state level.

Cuba-Ozone-CenterIn 1982, ozone was already used in Cuba in water disinfection and other environments. At that time it started doing research on medical applications of ozone in the National Center for Scientific Research (CNIC), the most experienced scientific institution in the country.
In 1988, the First National Congress on Ozone Applications was held, involving many and in 1990, the 1st Ibero Latin American Congress of Ozone Therapy took place.
In 1992 the Ozone Research Center was created by the CNIC Department of Ozone.
Since 2009 ozone therapy has been applied throughout all of Cuba according to the Ministerial Resolution 261 issued by the Ministry of Public Health.

Ozone Therapy Throughout the World
At present there are more than 40 national and international associations
that bring together the professionals that practice this therapy as well as indexed specialized journals, continuing training courses and ozone conferences. In the US it is the American Academy Ozone Therapy (AAOT). There are also over 26,000 ozone therapists throughout the world.

What is  (UV) Ultraviolet light?

Ultraviolet light is a naturally occurring component of sunlight. It falls in the region between visible light and X-Rays in the electromagnetic spectrum.  Generally, UV light is considered as falling between 100nm & 400nm in wave-length, however UV light in itself can be categorized even further into separate regions.  Of these UV regions, UVC is recognized as having significant germicidal properties. UVC light is however, almost entirely filtered out by the Earth’s atmosphere. As such, if we are to utilize the germicidal properties of UVC light, we have to artificially generate it here on Earth using commercially produced UV lamps.


Is UV light harmful?

UV light is good and necessary for our environment but too much exposure or exposure to intense rays can be detrimental.  UVA, UVB, and UVC can all damage collagen fibers and, therefore, accelerate aging of the skin.  Prolonged exposure to UVC light will also damage the eyes.

Used correctly however, UV light can be a very healthy, useful and effective tool.  

What types of ultraviolet (UV) light are used in your machine?

We use both UVA and UVC in both of our machines.  Blue light is also utilized in the Gorilla UV unit.


What is ozone?

Ozone, (O3), is an energized form of oxygen which contains three atoms of oxygen rather than the two atoms we normally breathe. Ozone is the second most powerful sterilizer in the world and can be used to destroy bacteria, viruses and odors. Interestingly ozone occurs quite readily in nature, most often as a result of lightning strikes that occur during thunderstorms. In fact the “fresh, clean, spring rain” smell that we notice after a storm most often results from the creation of ozone. However, we are probably most familiar with ozone from reading about the “ozone layer” that circles the planet above the earth’s atmosphere. Here ozone is created by the sun’s ultraviolet rays. This layer of ozone serves to protect us from the ultraviolet radiation.

How does ozone work?

The third oxygen atom of ozone makes it extremely reactive. This atom readily attaches itself to other molecules. When contaminants such as bacteria or viruses make contact with ozone, it creates a miniscule whole in the cell wall.  Once the cell wall is destroyed, the bacteria will be unable to survive.. This process is called oxidation. Ozone essentially reverts back to oxygen after it is used. This makes it a very environmentally friendly oxidant.

Isn’t ozone a pollutant?

Unlike stratospheric ozone, which forms naturally in the upper atmosphere and protects us from the sun’s harmful ultraviolet rays, ground-level (or tropospheric) ozone is created through the interactions of man-made (and natural) emissions of volatile organic compounds and nitrogen oxides in the presence of heat and sunlight. Cars and gasoline-burning engines are large sources of volatile organic compounds (VOCs). VOCs also come from consumer products such as paints, insecticides, and cleaners as well as industrial solvents and chemical manufacturing. Nitrogen oxides (NOx), the other chemical precursor of ozone, are produced whenever fossil fuels are burned and are primarily produced by motor vehicles and power plants.  The sun’s direct ultraviolet rays convert these emissions into ground-level ozone, which is unhealthy to breathe. 

How is ozone measured?

1)      Concentrations

  1. a.      Parts Per Million (ppm) – This is the most common method.  So if we were to have 1 ppm that would mean that for every million parts of gas, one of those would be ozone.  (Note: ppb = parts per billion)
  2. b.      Milligrams(mg/l) OR  Micrograms(ug/ml) of ozone per liter – indicates milligrams/micrograms of ozone in one liter of total volume (gas or liquid).

2)      Ozone  Production

  1. a.      Grams of ozone per hour (g/hr) – ozone output for a generator.  We can measure the concentration of ozone in g/m3, then when we calculate for flow rate with a measurement such as LPM (liters per minute), we can determine how many grams of ozone are produced in one hour of time.
  2. b.      Milligrams of ozone per hour (mg/hr) – same as grams but a smaller measurement.

What is a gamma?

A gamma is essentially another term for mg/l (milligram per liter).  It is a measurement of the concentration of the ozone.  Therefore, 1 gamma = 1 mg/l.

Is ozone safe?

Ozone is safe if used correctly. However, high concentrations of ozone in the air can cause health problems if proper safety measures are not taken.   OSHA, has stipulated that the safe allowable level of residual is 0.1 ppm (parts per million). Note that this permissible level is for continuous exposure throughout an entire 8 hour day.

If you start to cough when around ozone there is too much ozone in the air and measures should be taken to remove yourself from the location or properly ventilate while ensuring that the cause of ozone in the air is remedied.  Do keep in mind that it takes an extended period of time at very elevated levels to cause irritation to the lungs.

How is ozone produced?

There are basically two methods of producing ozone…ultraviolet and corona discharge. Corona discharge creates ozone by applying high voltage to a metallic grid sandwiched between two dielectrics. The high voltage passes through the dielectric to a grounded screen/plate and in the process, creates ozone from oxygen present in the chamber. Ultraviolet (UV) light creates ozone when a wavelength at 254 nm (nanometers) hits an oxygen atom. Both processes split oxygen molecules into single oxygen atoms (O). These atoms combine with another oxygen molecule (O2) to form ozone (O3).

How long does the ozone last?

The length of time that ozone exists in a given substance is directly related to the ability of that substance to help ozone maintain its chemical composition.  For example, ozone that is created in the air will immediately begin the process of reverting back to oxygen.  A basic rule of thumb is that the half life for ozone in the air is 30 about minutes.  This means that every 30 minutes, the amount of ozone will decrease by 1/2.  Higher temperatures cause it to break down even quicker.


70 degree Fahrenheit 

50 degree Fahrenheit 

Olive Oil

Holistic Pet Care Using UV Machine

Which UV machine is right for me?

It depends on whether you are treating large animals or just small animals.  With the Gorilla UV, you can treat both large (horses, cows…gorillas etc) and small (cats, dogs etc)  animals, while the Chimp UV is designed for animals up to about 200 lbs (including Chimps). 

When do I need to replace the bulbs in the UV machine?

We recommend replacing the bulb after 1,000 hrs. of use.  This is far less than the stated bulb life and will ensure peak energy performance. 

Do the machines require maintenance?

Both our UV and Ozone machines are virtually maintenance free.  Replacing fuses or bulbs may be necessary after the machine has been used for a period of time. 

How many times can I use the cuvette before replacing it?

The cuvette is a sterile single use product.  Re-sterilization of the cuvette is up to the discretion of the clinician

What is O3UV?

O3UV is the combination of ozone (O3) and UBI (UV).  More specifically, it is the combination of MAHT and UBI.  Basically, blood is withdrawn, diluted with saline, infused with ozone and then fed past the UV light and back into the patient.

Why combine ozone and UBI?

World-renown ozone trainer, clinician and author Frank Shallenberger pioneered this combination treatment at his clinic in Nevada.  Dr. Shallenberger saw that ozone had some positive effects that UBI didn’t and vice-versa.  He was a firm believer in both therapies and because they both are oxidative, germicidal and have almost no side effects, he began to run the ozone treated blood through the UBI machine before returning it to the patient.  The effects were better than ever and the ozone/UBI treatment was born.  Since that time there have been thousands of treatments performed with stunning results.  Even more recently, we have come up with the term O3UV to refer to what we believe to be one of the better therapies available.

Can I use Ozone and UBI separately?

Absolutely.  Both ozone and UBI are therapies that work very even when only one or the other is used.  If you simply choose to purchase one machine or the other, you will not be disappointed, however, our recommendation is that you incorporate both into your practice. 

Why not just use conventional treatments?

Although modern medicine has greatly benefited societies as a whole, there are also ways in which it has greatly hampered overall health.  There is more to healing than just giving medicine or cutting things out.  Often medicines just mask symptoms and do little to treat the root causes.  O3UV works with the immune system stimulating the body to “heal itself” which is truly the ultimate and only real way to healing. 

What is holistic vet medicine?

Holistic (a.k.a. Integrative or Complementary) Veterinary Medicine is the examination and diagnosis of an animal, considering all aspects of the animal’s life and employing all of the practitioner’s senses, as well as the combination of conventional and alternative (or complementary) modalities of treatment.  When a holistic veterinarian sees a pet, besides giving it a comprehensive physical examination, he/she wants to find out all about its behaviors, distant medical and dietary history, and its environment including diet, emotional stresses, and other factors. 

Is O3UV better suited for a large animal as opposed to smaller pets?

No.  Ozone is equally effective for large or small animals.

How much blood do I have to draw?

The total amount of fluid treated depends upon the size of the animal, but a good rule of thumb is about 1.5ml of blood saline mix for every pound of body weight.  The saline to blood ratio is 4:1 (four parts saline to one part blood).  So for example, a 50 lb dog would have 15ml of blood drawn and mixed with 60mls of saline for a total of 75mls.  ozone is then mixed at a 1:1 ratio with the amount of blood that was drawn. 

Can we take human studies and apply them to animals?

The answer is yes.  Most studies begin with animals and progress to the human level and it is no different with ozone and UBI.  There are many studies which show the efficacy and safety for both man and animals. 

How do I know how much treatment to give?

Of course you need to follow the protocols for any given therapy, but the number of therapy sessions required to heal a specific illness will vary depending on the animal, overall health, whether it is chronic or acute and other factors as well.  Acute illnesses usually can be remedied within 1-3 treatments while chronic illnesses may take up to 10 – 12.  You should see a response from most illnesses within 4-5 treatments.  If no response is seen by that point, the clinician should evaluate whether O3UV will be effective method of treatment for the given animal/illness.

What is UBI?

UBI or Ultraviolet Blood Irradiation is known by a number of names including, Bio-Photonic therapy, Photo-oxidative therapy and others.  It has been around since the 1920’s during which time there have been over 200 clinical studies that have been published.

In a nutshell, a small amount of blood is withdrawn from the patient, diluted with saline and then exposed to the ultraviolet light as it is re-infused back to the patient.

Why do you use those particular bands of light?


How does treating such a small amount of blood have such a wonderful effect? 

A UBI treatment can be understood, at least when referring to the effects of UVC, as an auto-vaccine.  Very simply, the small amount of blood containing the inactivated bacteria or virus is returned to the patient enabling its immuno-defense system to recognize and attack pathogens all throughout the body.  This kicks off a “cascade of healing” as the patients intricately designed immune system rises to the occasion and self-healing begins. 

Is UBI safe?

There has historically been a complete absence of harmful effects, either immediate or delayed, when UBI is used properly.  This has allowed clinical investigators to use this procedure over a period of twenty years and more on a single patient.  Some patients may experience flushing, slight “flu-like” symptoms, a temporary rise in temperature or chills

Ozone Therapy Machine in Vet Medicine

What does ozone do in the body?

  1. Ozone is a potent regulator of the immune system.
  2. Ozone stimulates increased uptake of oxygen.
  3. Ozone improves circulation.
  4. Ozone is a powerful mitochondrial stimulant.
  5. Ozone increases antioxidant protection.

When healthy, the body is able to rid itself of toxins which would otherwise accumulate to harmful levels through the process of oxidation.  Oxidation is the process wherein a substance is changed due to the effects of oxygen upon it.  Oxidation breaks the toxins down into carbon dioxide and water, and eliminates them from the body.  However, if the necessary oxygen is not available within the body (whether because of lack of exercise, environmental pollution, poor diet, smoking or improper breathing) our bodies cannot eliminate these toxins adequately and a toxic reaction can occur.  In minor cases, a toxic buildup can lead to fatigue, while a wide range of diseases can result when poor oxygenation is chronic.  Why does ozone work so well? Oxygen plays the most vital role for an optimum immune system and maintaining health. Along with food, it is the primary nutrient that cells use to generate energy for all of their functions. Oxygen regulates all activities of our bodies and also the bodies of our pets . Our ability to think, feel, and act comes from the energy created by the oxygen. Did you know that approximately 90% of our energy originates from oxygen?  The oxygen we breath is our most vital element for good health!  If ozone therapy is that effective why is it not more popular?

Ozone therapy is very accepted and used in Germany where there are over 11,000 Drs doing over 1 million treatments per year.  World wide over 30,000 Drs use ozone as an effective and safe therapy.  What are the different ways in which ozone can be administered to animals? As a solo therapy, ozone can be administered in the following ways:

  1. DIV – Ozone gas is injected directly into the vein.
  2. MAHT – Blood is withdrawn and infused with ozone and then re-infused into the animal.
  3. MAHT – Blood (1-5ml) is withdrawn, infused with ozone and injected intra-muscularly
  4. Insufflation – Gas is infused through a body orphus
  5. Limb Bagging – Gas is bubbled through a liquid and into a bag that is sealed around an area of the body.
  6. Topically -With the use of oils (i.e. olive, hemp or jojobo).
  7. Water or saline – Ozonated water can be drunk and saline can be administered through an IV.  

Can ozone therapy be administered at home? It is possible to administer some of these treatments at home with the proper training. Ozone oils are available for home use.  Does ozone therapy really cure as many diseases as they claim? This is a tough question to answer, but ozone’s track record speaks for itself.  Because oxygen is such a rudimentary necessity, ozone is able to have a positive effect on a variety of diseases.